High-throughput sequencing has provided a myriad of genetic data for thousands of organisms. Computational analysis of one data type, expressed sequence tags (ESTs) yields insight into gene expression, alternative splicing, tissue specificity gene...
Due to the complex nature of interactions at the genomic level as well as the large number of proteins present in an organism, understanding the functions of various genes that are expressed is essential. Creating an analysis pipeline for Expressed...
Short-term memory; Memory--Effect of sound on; Memory--Effect of noise on; Speech
The Irrelevant Speech Effect (ISE) is a phenomenon in which the presentation of
auditory materials (typically speech) impairs the serial recall of visually presented
materials, either digits or letters. Although this effect has been replicated in a...
Radio frequency allocation; Wireless communication systems
Recently, the need for wireless and mobile communications has grown tremendously and it is expected that the number of users to be supported will increase with high rates in the next few years. Not only the number of users, but also the required...
Heart--Diseases--Diagnosis; Diagnostic imaging; Magnetic resonance imaging; Imaging systems in medicine
Many cardiac diseases, such as myocardial ischemia, secondary to coronary artery disease, may be identified and localized through the analysis of cardiac deformations. Early efforts for quantifying ventricular wall motion used surgical implantation...
Current high-throughput gene expression experiments have a straightforward design of examining
the gene expression of one group or condition relative to that of another. The data is typically
analyzed as if they represent strictly intracellular...
For complex classification systems, data is usually gathered from multiple sources of information that have varying degree of reliability. In fact, assuming that the different sources have the same relevance in describing all the data might lead to...
For more than a hundred million years, male plethodontid salamanders have
utilized non-volatile, proteinaceous courtship pheromones to regulate female mating
receptivity and promote mating success. These pheromones - which are delivered...
Eating disorders in children; Behavior modification
Feeding disorders occur when a child rejects significant amounts of food or drink. Food refusals are maintained by factors which may be behavioral based, due to structural impairments, or a combination of the two. One of the first steps to...
This thesis begins by reviewing human N-acetyltransferases (Chapter I), then outlines experiments involving human hepatocytes and rat N-acetyltransferases (Chapters II-V). These experiments facilitated the development of a dissertation project...
The thesis project, Gene Database, was done to create a way for the bioinformatics research group at the University of Louisville to have access to GenBank EST information in the form of a database. This database allows for a programmable front end...
While investigating estrogen response element (ERE) binding properties of Erα in de-identified human breast cancer extracts, additional proteins were observed that recognized ERE sequences (ERE-BP). In order to unravel the apparent role of these...
Brief Overview of the Problem: The Environmental Protection Agency (EPA), a government funded agency, provides both legislative and judicial powers for emissions monitoring in the United States. The agency crafts laws based on self-made regulations...
G-quadruplexes are thought to have biological importance, with studies based on small molecule interactions and quadruplex-interactive antibodies demonstrating their potential for formation in vivo. One potential biological function of quadruplex...
Crossover design is a type of longitudinal study with each subject receiving different treatments in different time periods. It has been used frequently in the pharmaceutical industry and other medical fields to investigate the safety and efficacy...
Here, I address the function of the carboxy-terminal hydrophobic helix of the pro-apoptotic protein Bax. There has been considerable controversy as to whether this sequence is required for the targeting and insertion of Bax into the mitochondrial...
Congenital stationary night blindness 1 (CSNBl) is a genetic disorder characterized in humans by night blindness, low visual acuity and myopia. CSNB 1 is caused by defects in genes that are involved in signaling between photoreceptors and...
The current status of our knowledge of synaptic plasticity comes largely from studies of the hippocampus and the context of learning and memory. We remain largely ignorant of plasticity in other neural systems and contexts. The molecular basis of...
Background: Helicobacter pylori (Hp) establishes life-long gastric infection in billions of humans, and is often responsible for diseases such as peptic ulcer and gastric cancer. Cumulative actions of genetic drift and natural selection over...
Prostate--Cancer--Diagnosis; Chemical detectors; Biosensors
Prostate cancer is the most common type diagnosed among men in the United States.
Random oligodeoxynucleotide (ODN) libraries are used to generate DNA aptamers by
systematic evolution of ligands by exponential enrichment (SELEX). We explored...
