N-acetyltransferase 1 (NAT1) is a phase II metabolic enzyme responsible for the biotransformation of aromatic and heterocyclic amine carcinogens such as 4-aminobiphenyl (ASP). NAT1 catalyzes N-acetylation of arylamines as well as the 0-acetylation...
Interactions between chemokines and their receptors are involved in organ- specific homing and propagation of metastatic breast cancer (BrCa) cells. BrCa cells express higher levels of CXCR4 and CXCR7 mRNA and cell surface protein, than normal...
Cancer is the second-most leading cause of death in the United States, with 1.66 million new cases expected to be diagnosed and over 580,000 Americans expected to die of cancer in 2013 alone. (American Cancer Society 2013) Current treatments result...
While investigating estrogen response element (ERE) binding properties of Erα in de-identified human breast cancer extracts, additional proteins were observed that recognized ERE sequences (ERE-BP). In order to unravel the apparent role of these...
Current high-throughput gene expression experiments have a straightforward design of examining
the gene expression of one group or condition relative to that of another. The data is typically
analyzed as if they represent strictly intracellular...
Epithelial Ovarian cancer (EOC) is the leading cause of gynecological cancer death in the USA. Recurrence rates are high after front-line platinum chemotherapy and most patients eventually die from platinum-resistant disease. P53 plays an important...
The most important non-protein nitrogenous constituents of milk are urea, creatinine, creatine, and uric acid. Urea is the diamide of carbonic acid and is the chief end-product, so far as nitrogen is concerned, of the physiological metabolism of...
O -linked β-N-acetylglucosamine ( O -GlcNAc) is an inducible, dynamically cycling, and reversible post-translational modification of serine/threonine amino acid residues of nucleocytoplasmic and mitochondrial proteins. O -GlcNAc transferase (OGT)...
DNA microarrays--Statistical methods; Gene expression--Statistical methods
Data derived from gene expression microarrays are frequently used to identify candidate genes which can characterize and distinguish between two biological phenotypes. A key step in this process is the selection of an appropriate test statistic to...
Computer-aided minimally invasive surgery (MIS) has progressed significantly in the last decade and it has great potential in surgical planning and operations. To limit the damage to nearby healthy tissue, accurate modeling is required of the...
Inflammation is now recognized as an important factor in several age-related diseases such as arthritis, atherosclerosis, multiple sclerosis and diabetes. In each case, sub-clinical chronic inflammation occurs over years and leads to progressive...
From a computerized image analysis prospective, early diagnosis of lung cancer involves detection of doubtful nodules and classification into different pathologies. The detection stage involves a detection approach, usually by template matching,...
T lymphopoiesis has been an intense focus of immunological research since the discovery of the major histocompatibility complex (MHC) and T cell mediated transplant rejection. Additionally, researchers have long appreciated the role of T cells, and...
Periodontitis is a chronic, destructive inflammatory disease of the supporting
tissues of the teeth with a high prevalence among adults. While the complete
pathogenesis of periodontitis remains unclear, it is initiated and sustained by...
Tobacco smoke is a strong and independent risk factor for several chronic systemic diseases and also increases susceptibility to a multitude of bacterial infections, including periodontal infections. Periodontitis is a chronic inflammatory disease...
Hypoxia is characterized by an inadequate oxygen supply to the tissues in
proportion to their metabolic needs, and is a primary factor in traumatic CNS
injury, strokes, cardiopulmonary diseases, and obstructive sleep apnea. The
cAMP-dependent...
The purpose of this dissertation was to demonstrate that a new method of direct intracellular energy delivery was effective in maintaining viability of in vitro Human Umbilical Vein Endothelial Cells (HUVECs) when exposed to chemical ischemia for...
This thesis begins by reviewing human N-acetyltransferases (Chapter I), then outlines experiments involving human hepatocytes and rat N-acetyltransferases (Chapters II-V). These experiments facilitated the development of a dissertation project...
